[Effect of Aloe emodin on invasion and metastasis of high metastatic breast cancer MDA-MB-231 cells]
| Autor: | Zhen-Hui, He, Yue-Qun, Huang, Shan-Fan, Weng, Yao-Rong, Tan, Tai-Ping, He, Yan-Mei, Qin, Nian-Ci, Liang |
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| Rok vydání: | 2014 |
| Předmět: |
Mice
Inbred BALB C Lung Neoplasms Dose-Response Relationship Drug Anthraquinones Breast Neoplasms Antineoplastic Agents Phytogenic Xenograft Model Antitumor Assays Cell Movement Cell Line Tumor Cell Adhesion Animals Humans Female Neoplasm Invasiveness Aloe Neoplasm Metastasis Neoplasm Transplantation Cell Proliferation |
| Zdroj: | Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials. 36(9) |
| ISSN: | 1001-4454 |
| Popis: | To investigate the effect of Aloe emodin (AE) on the invasive and metastatic abilities of human high metastatic breast cancer MDA-MB-231 cells.MTT assay was used to evaluate the viability of MDA-MB-231 cells after treated with AE for 6 h and 24 h. The adhesive potential of MDA-MB-231 cells to FN and LN was tested by cell-matrix adhesion assay. The effect of AE on invasion of MDA-MB-231 cells was measured by Transwell chamber assay. Scratch wound healing assay was applied to determine the effect on migration of MDA-MB-231 cells. The effect of AE on MDA-MB-231 lung metastasis was determined on an experimental metastatic model.80 micromol/L AE significantly inhibited the invasion, adhesion to FN, LN of MDA-MB-231 cells in vitro, the inhibitory rates were (52.98 +/- 5.46)%, (34.99 +/- 2.63)%, (28.73 +/- 7.00)%, respectively. After 24 h treatment, AE significantly inhibited the migration of MDA-MB-231 cells. The number and volume of lung metastatic nodules formed by MDA-MB-231 cells after 80 micromol/L AE 24 h treatment were decreased compared with control group.AE can suppress the metastasis of MDA-MB-231 cells. Their mechanisms may be related to the inhibition of the capabilities of invasion and migration of MDA-MB-231 cells. |
| Databáze: | OpenAIRE |
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