Lack of correlation of functional scintigraphy with (99m)technetium-methoxyisobutylisonitrile with histological necrosis following induction chemotherapy or measures of P-glycoprotein expression in high-grade osteosarcoma
| Autor: | R, Gorlick, A C, Liao, C, Antonescu, A G, Huvos, J H, Healey, R, Sowers, M, Daras, E, Calleja, L H, Wexler, D, Panicek, P A, Meyers, S D, Yeh, S M, Larson |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Technetium Tc 99m Sestamibi Osteosarcoma Adolescent Reverse Transcriptase Polymerase Chain Reaction Bone Neoplasms Pilot Projects Immunohistochemistry Bone and Bones Gene Expression Regulation Neoplastic Necrosis Humans Female ATP Binding Cassette Transporter Subfamily B Member 1 RNA Neoplasm Child Radionuclide Imaging |
| Zdroj: | Clinical cancer research : an official journal of the American Association for Cancer Research. 7(10) |
| ISSN: | 1078-0432 |
| Popis: | In osteosarcoma, some studies have suggested P-glycoprotein expression is a prognostic factor. The clearance of (99m)technetium hexakis-2-methoxyisobutylisonitrile ((99m)Tc-MIBI) has been used in some tumor systems as an in vivo measure of P-glycoprotein-mediated efflux. In this study we explored the correlation between (99m)Tc-MIBI clearance and histological necrosis following induction chemotherapy and P-glycoprotein expression in osteosarcoma. The primary tumors of 20 patients with high-grade osteosarcoma were imaged at diagnosis with (99m)Tc-MIBI, and the uptake ratios and biological half-lives were calculated. P-Glycoprotein expression in the tumor tissue was determined immunohistochemically and by measuring mRNA expression of the multidrug resistance-1 gene. The histological necrosis following induction chemotherapy was assessed by the Huvos grading system. The biological half-life of (99m)Tc-MIBI ranged from 1.4 to 52.5 h. Seven of the 20 tumor samples had a favorable extent of necrosis following induction chemotherapy. The (99m)Tc-MIBI half-life and uptake ratio showed no correlation with histological necrosis following induction chemotherapy. The (99m)Tc-MIBI half-life and uptake ratio did not correlate with either measure of P-glycoprotein expression. The results of this pilot study indicate that (99m)Tc-MIBI imaging is not an effective predictor of histological necrosis following induction chemotherapy in high-grade osteosarcoma. (99m)Tc-MIBI imaging did not correlate with measures of P-glycoprotein expression in the tumor tissue. |
| Databáze: | OpenAIRE |
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