Upgrading end-of-line residues of the red seaweed
| Autor: | S, Tůma, J K, Izaguirre, M, Bondar, M M, Marques, P, Fernandes, M M R, da Fonseca, M T, Cesário |
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| Rok vydání: | 2020 |
| Předmět: |
MSG
monosodium glutamate NREL National Renewable Energy. Laboratory P3HB poly-3-hydroxybutyrate AHG anhydro-L-galactose Fr Froude number CBU CelloBiase Unit Halomonas boliviensis Seaweed residues FPU Filter Paper Unit Gelidium sesquipedale HMF 5-hydroxymethyl furfural NABH neoagarobiose hydrolase Macroalgae residues CDW cell dry weight ComputingMethodologies_COMPUTERGRAPHICS dw basis dry weight basis Mw molecular weight KNU Kilo Novo alpha-amylase Unit H. boliviensis Halomonas boliviensis FID flame ionization detector AOAC Association of Official Agricultural Chemists BHU (2) Biomass Hydrolysis Unit AGU AmyloGlucosidase Unit Poly-3-hydroxybutyrate G. sesquipedale Gelidium sesquipedale Research Article Waste seaweed |
| Zdroj: | Biotechnology Reports |
| ISSN: | 2215-017X |
| Popis: | Graphical abstract Highlights • Residues of red seaweed Gelidium after phycocolloid extraction still contain 44 % of upgradable carbohydrates. • Hydrolysis of Gelidium cabohydrates to monosaccharides yielded 30 % (w/w) glucose per g dry biomass. • Hydrolysates from Gelidium residues are suitable C-sources for P3HB production by Halomonas boliviensis. • Halomonas boliviensis accumulated 41 % (w/w) of P3HB based on the algal hydrolysates. • The outlined process adds value to waste seaweed and promotes circular economy. Agar extraction from Gelidium and Gracilaria red seaweed species produces hundred thousand ton of carbohydrate-rich residues annually. Gelidium sesquipedale waste biomass obtained after agar extraction, still contained 44.2 % w/w total carbohydrates (dry-weight basis). These residues were biologically up-graded to poly-3-hydroxybutyrate (P3HB) after saccharification of their carbohydrate fraction to simple sugars. A combined hydrolysis treatment using sulfamic acid followed by enzymatic hydrolysis with cellulases produced a glucose-rich hydrolysate with a negligible content of inhibitors. With this treatment a sugar yield of circa 30 % (g glucose/g biomass) was attained. The algal hydrolysates were assessed as carbon source for the production of P3HB by the halotolerant bacteria Halomonas boliviensis. A cell concentration of 8.3 g L−1 containing 41 % (w/w) of polymer and a yield (YP/S) of 0.16 gpolymer/gglucose were attained in shake flask assays. In this work, cellulose-rich seaweed waste was shown to be an upgradable, sustainable source of carbohydrates. |
| Databáze: | OpenAIRE |
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