Perforin-deficient CD8+ T cells provide immunity to Listeria monocytogenes by a mechanism that is independent of CD95 and IFN-gamma but requires TNF-alpha
| Autor: | D W, White, J T, Harty |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Cytotoxicity
Immunologic Mice Knockout Pore Forming Cytotoxic Proteins Mice Inbred MRL lpr Membrane Glycoproteins Perforin Tumor Necrosis Factor-alpha Bacterial Toxins Epitopes T-Lymphocyte CD8-Positive T-Lymphocytes Cytotoxicity Tests Immunologic Adoptive Transfer Listeria monocytogenes Mice Inbred C57BL Hemolysin Proteins Interferon-gamma Mice Animals Listeriosis fas Receptor Heat-Shock Proteins Spleen |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 160(2) |
| ISSN: | 0022-1767 |
| Popis: | CD8+ T cells are effective mediators of immunity against Listeria monocytogenes, but the mechanisms by which they provide antilisterial immunity are poorly understood. CD8+ T cells efficiently lyse target cells in vitro by at least two independent pathways. To test the hypothesis that CD8+ T cell-mediated immunity to L. monocytogenes is dependent on perforin or CD95 (Fas, Apo-1), we used C57BI/6 (B6) and perforin-deficient (PO) mice to generate CD8+ T cell lines specific for the L. mono cytogenes-encoded Ag listeriolysin O (LLO). Both lines specifically produce IFN-gamma and TNF-alpha, and mediate target cell lysis in vitro. Cytolysis mediated by the PO-derived CD8+ T cell line is delayed relative to the B6-derived line and is completely inhibited by anti-CD95 Abs. In vivo, PO-derived CD8+ T cells provide specific antilisterial immunity in B6 hosts, CD95-deficient hosts, and IFN-gamma-depleted hosts. However, PO-derived CD8+ T cells fail to provide antilisterial immunity in hosts depleted of TNF-alpha. These results indicate that single Ag-specific CD8+ T cells derived from PO mice can mediate antilisterial immunity by a mechanism that is independent of CD95 or IFN-gamma, but requires TNF-alpha. |
| Databáze: | OpenAIRE |
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