| Autor: |
M, Daffé, M A, Lanéelle, C, Asselineau, V, Lévy-Frébault, H, David |
| Jazyk: |
francouzština |
| Rok vydání: |
1983 |
| Předmět: |
|
| Zdroj: |
Annales de microbiologie. (2) |
| ISSN: |
0300-5410 |
| Popis: |
A simple and quantitative method for the alkaline hydrolysis of fatty acid derivatives occurring in the lipids of mycobacteria is described. After methylation, the lipidic mixtures were chromatographed on a thin layer of silicagel. The contents in mycolates and secondary alcohols allowed the distinction of 9 groups among the 27 species studied. Such an analysis is generally insufficient to identify a species, but its discriminating power differs from that of other commonly-used methods. Complementary tests chosen according to each particular situation are necessary, including vapour phase chromatography applied to the same lipidic mixture as that used for thin-layer chromatography. Coupling of the two chromatographic methods would allow the recognition of 22 groups among the 27 species of mycobacteria studied. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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