A simple, three-step procedure for the large scale purification of DNA ligase from a hybrid lambda lysogen constructed in vitro

Autor:	S M, Panasenko, R J, Alazard, I R, Lehman
Rok vydání:	1978
Předmět:	Polynucleotide Ligases DNA Ligases Escherichia coli Methods Coliphages Lysogeny
Zdroj:	The Journal of biological chemistry. 253(13)
ISSN:	0021-9258
Popis:	A simple three-step procedure for the large scale purification of DNA ligase has been developed. THe source of enzyme is a strain of Escherichia coli with a hybrid lambda prophage constructed in vitro that bears the ligase overproducing gene lop 11 lig+ (Panasenko, S., Cameron, J., Davis, R. W., and Lehman, I. R. (1977) Science 196, 188-189). The procedure yields homogeneous enzyme in approximately 40% yield.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::5a876a8ba9c46d9bdb63d73ca2b93f20 https://pubmed.ncbi.nlm.nih.gov/350879 Zobrazit plný text záznamu