C-terminal amidation of calcitonin by carboxypeptidase Y catalyzed transpeptidation with a photocleavable nucleophile

Autor:	D B, Henriksen, M, Rolland, M H, Jakobsen, O, Buchardt, K, Breddam
Rok vydání:	1992
Předmět:	Calcitonin Hydrazines Photochemistry Molecular Sequence Data Glycine Amino Acid Sequence Carboxypeptidases Hydrogen-Ion Concentration Amides Nitrobenzenes
Zdroj:	Peptide research. 5(6)
ISSN:	1040-5704
Popis:	The C-terminal amidation of calcitonin represents an important technological problem. A method using a serine carboxypeptidase-catalyzed transpeptidation reaction in combination with photochemical cleavage to give the warranted peptide amide is described. The overall yield is higher than 95%.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::5a84b5965b2486cd01db022cac7c4917 https://pubmed.ncbi.nlm.nih.gov/1493358 Zobrazit plný text záznamu