| Autor: |
P Y, Marquet, A, Daver, R, Sapin, B, Bridgi, J P, Muratet, D J, Hartmann, F, Paolucci, B, Pau |
| Rok vydání: |
1996 |
| Předmět: |
|
| Zdroj: |
Clinical chemistry. 42(2) |
| ISSN: |
0009-9147 |
| Popis: |
Five monoclonal antibodies (MAbs) directed against antigenic domains on thyroglobulin (Tg) not recognized by most anti-Tg human autoantibodies (aAbs) have been used to develop an improved IRMA for serum Tg with a limit of detection of 0.2 micrograms/L. Samples are incubated for 3 h in tubes coated with four anti-Tg MAbs. After washing, the tubes are incubated with the tracer MAb for 20 h at room temperature. Dilution and reproducibility tests demonstrated assay reliability. Tests performed on samples with (n = 361) or without (n = 283) aAbs showed that the TG IRMA Pasteur is largely independent of the marked interference generally caused by aAbs. These results were confirmed with an extended population of 2759 samples. For a cutoff of 1 micrograms/L, sensitivity and specificity were 0.97 and 1, respectively, in a follow-up of differentiated thyroid carcinoma in patients treated by total thyroidectomy. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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