| Autor: |
Monika, Majewska-Szczepanik, Paulina, Kowalczyk, Dominika, Biała, Katarzyna, Marcińska, Anna, Strzępa, Dorota, Woźniak, Piotr, Sura, James, Pearson, Li, Wen, Marian, Szczepanik |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Pharmacological reports : PR. 70(4) |
| ISSN: |
2299-5684 |
| Popis: |
Cyclophosphamide (CY) is one of the most widely used alkylating agents in the treatment of various cancers and some autoimmune diseases. Numerous reports suggest that CY exerts immunoregulatory effects. Animal studies have shown CY affects contact sensitivity (CS) response by depleting CD4Adoptive cell transfer of CS was used to examine immunomodulating properties of peritoneal Mf from CY-treated mice. Isolation of peritoneal Mf from animals that were (Mf-CY) or were not (Mf) treated with CY were cultured to identify cytokine repertoire. Further, we assessed spleen cell (SPLC) cytokine production following immunization with trinitrophenyl-conjugated Mf from donors treated (TNP-Mf-CY) or non-treated (TNP-Mf) with CY.In vitro experiments identified that Mf-CY produce more IL-6, TNF-α and TGF-β than naïve Mf. Further, immunization with peritoneal TNP-Mf-CY induces CD4Our results show that in vivo treatment with CY influences mouse peritoneal Mf to induce CD4+ Tcs cells that protect CS-effector cells from suppressive signals of Ts cells. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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