| Popis: |
The study aimed at application of a high performance liquid chromatography (HPLC) with UV detector for selenium speciation in samples of mineral and well waters in Poland. Basic solutions 1000 microg/l SeO3(2-) and SeO4(2-) were prepared by dissolving 2.190 g of reagent Na2SeO3 and 2.393 g Na2SeO4 in 1000 ml of deionised water, respectively. Recent standards SeO3(2-) and SeO4(2-) (single or mixed) were prepared daily. Chromatographic separations were obtained on a column with reversed phase of C18, 250*4 mm, 5 microm ODS. As mobile phase mixture of 5 mmol/l phosphate tetrabutylammonium, 50/50 water/methanol at pH 3.4 was used. Flow rate of mobile phase was 1 ml/min and detector was set on lambda = 210 nm. Injection volumes were 5 microl. The average concentration of Se(IV) was 4.10 microg/l and Se(VI) - 4.27 microg/l and Se(II) - 0.54 microg/l although the differences between water samples originating from various sources were relatively high. The developed method can be therefore recommended for determination of Se in samples of mineral water. |
