Overexpression of IL-6 by gene transfer stimulates IL-8-mediated invasiveness of KYSE170 esophageal carcinoma cells
| Autor: | Shinichi, Okamura, Hitoshi, Fujiwara, Masayuki, Yoneda, Akinobu, Furutani, Momoko, Todo, Atsushi, Ikai, Hiroyuki, Tada, Hiroko, Okamura, Seiji, Umehara, Atsushi, Shiozaki, Takeshi, Kubota, Shuhei, Komatsu, Daisuke, Ichikawa, Kazuma, Okamoto, Toshiya, Ochiai, Eigo, Otsuji |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Vascular Endothelial Growth Factor A
Esophageal Neoplasms Hepatocyte Growth Factor Interleukin-6 Reverse Transcriptase Polymerase Chain Reaction Blotting Western Genetic Vectors Interleukin-8 Gene Transfer Techniques Enzyme-Linked Immunosorbent Assay Real-Time Polymerase Chain Reaction Cell Movement Carcinoma Squamous Cell Tumor Cells Cultured Humans Neoplasm Invasiveness RNA Messenger RNA Small Interfering Cell Proliferation |
| Zdroj: | Anticancer research. 33(4) |
| ISSN: | 1791-7530 |
| Popis: | Interleukin-6 (IL-6) has been associated with disease progression and poor prognosis in esophageal carcinoma. The aim of this study was to investigate the possible influence of IL-6 on the biological activities of esophageal carcinoma cells in terms of invasiveness. The human esophageal carcinoma cell line, KYSE170, was transfected with a plasmid vector expressing IL-6, and a stable transfectant overexpressing IL-6 was established. Invasiveness was evaluated by an invasion assay and compared between IL-6 and control transfectants. The invasiveness of the IL-6 transfectant was significantly higher than that of the control transfectant, and was significantly reduced by IL-6-specific siRNA. In reverse transcriptase-polymerase chain reaction (RT-PCR) analysis, IL-8 expression was significantly higher in the IL-6 transfectant than in the control transfectant, whereas the expression of Hepatocyte growth factor (HGF) and Vascular endothelial growth factor (VEGF) was not different. IL-8 expression in the IL-6 transfectant was significantly inhibited by IL-8-specific siRNA, whereas IL-6 expression was not. In addition, the invasiveness of the IL-6 transfectant was significantly reduced by IL-8-specific siRNA. These results indicate that the overexpression of IL-6 increases the invasiveness of KYSE170 esophageal carcinoma cells and IL-6-induced IL-8 plays a predominant role in increasing invasiveness. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|