| Popis: |
Highly purified Fc receptor-negative T lymphocytes were obtained by filtration of human blood mononuclear cells through Ig–anti-Ig columns. Monocyte-enriched cells were isolated by density centrifugation in bovine serum albumin solutions. The proliferative in vitro response of the purified T lymphocytes was investigated with and without the addition of monocyte-enriched cells, after stimulation by allogeneic cells, phytohaemagglutinin (PHA), pokeweed mitogen (PWM), purified protein derivative (PPD), Candida albicans, Staphylococcus aureus, E. coli and tetanus toxoid. |
