Effect of decoy-oligodeoxynucleotides on expression of inflammation mediators in pMPhi cells from rats
| Autor: | Hua, Guo, Hua-ping, Liang, Feng-lin, Lu, Xiang, Xu, Bin, An, Tian-zhi, Hao, Fu-lLong, Wang, Wen-jun, Yang |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Male
Base Sequence Dose-Response Relationship Drug Interleukin-6 Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha Molecular Sequence Data NF-kappa B Sensitivity and Specificity Interleukin-10 Rats Random Allocation Oligodeoxyribonucleotides Reference Values Animals Female RNA Messenger Inflammation Mediators Rats Wistar Cells Cultured |
| Zdroj: | Chinese journal of traumatology = Zhonghua chuang shang za zhi. 6(6) |
| ISSN: | 1008-1275 |
| Popis: | To study the effect of decoy-oligodeoxynucleotides (decoy-ODNs) in dumbbell shape with the oligodeoxynucleotide sequence similar to nuclear factor kappa B (NF-kappaB) cis-elements on expression of inflammation mediators in pMPhi cells from rats.With carriers of cationic liposomes, decoy-ODNs were transfected into pMPhi cells of rats. Then the inhibiting effects of the decoy-ODNs on tumor necrosis factor alpha (TNFalpha), interleukin-6 (IL-6) and IL-10 were analyzed.Decoy-ODNs could decrease the expression of TNFalpha and IL-6 in dose-dependent fashion but had weaker inhibiting effect on IL-10.Decoy-ODNs targeting NF-kappaB can decrease the expression of inflammatory mediators in pMPhi cells from rats. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|