Gene expression profiling of connective tissue growth factor (CTGF) stimulated primary human tenon fibroblasts reveals an inflammatory and wound healing response in vitro
| Autor: | Axel, Seher, Joachim, Nickel, Thomas D, Mueller, Susanne, Kneitz, Susanne, Gebhardt, Tobias Meyer, ter Vehn, Guenther, Schlunck, Walter, Sebald |
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| Rok vydání: | 2010 |
| Předmět: |
Inflammation
Extracellular Matrix Proteins Wound Healing Chemokine CXCL6 Interleukin-6 Reverse Transcriptase Polymerase Chain Reaction Chemokine CXCL1 Gene Expression Profiling Interleukin-8 Connective Tissue Growth Factor Fibroblasts In Vitro Techniques Recombinant Proteins Gene Expression Regulation Humans Intercellular Signaling Peptides and Proteins Proteoglycans Research Article |
| Zdroj: | Molecular Vision |
| ISSN: | 1090-0535 |
| Popis: | Purpose The biologic relevance of human connective tissue growth factor (hCTGF) for primary human tenon fibroblasts (HTFs) was investigated by RNA expression profiling using affymetrixTM oligonucleotide array technology to identify genes that are regulated by hCTGF. Methods Recombinant hCTGF was expressed in HEK293T cells and purified by affinity and gel chromatography. Specificity and biologic activity of hCTGF was confirmed by biosensor interaction analysis and proliferation assays. For RNA expression profiling HTFs were stimulated with hCTGF for 48h and analyzed using affymetrixTM oligonucleotide array technology. Results were validated by real time RT–PCR. Results hCTGF induces various groups of genes responsible for a wound healing and inflammatory response in HTFs. A new subset of CTGF inducible inflammatory genes was discovered (e.g., chemokine [C-X-C motif] ligand 1 [CXCL1], chemokine [C-X-C motif] ligand 6 [CXCL6], interleukin 6 [IL6], and interleukin 8 [IL8]). We also identified genes that can transmit the known biologic functions initiated by CTGF such as proliferation and extracellular matrix remodelling. Of special interest is a group of genes, e.g., osteoglycin (OGN) and osteomodulin (OMD), which are known to play a key role in osteoblast biology. Conclusions This study specifies the important role of hCTGF for primary tenon fibroblast function. The RNA expression profile yields new insights into the relevance of hCTGF in influencing biologic processes like wound healing, inflammation, proliferation, and extracellular matrix remodelling in vitro via transcriptional regulation of specific genes. The results suggest that CTGF potentially acts as a modulating factor in inflammatory and wound healing response in fibroblasts of the human eye. |
| Databáze: | OpenAIRE |
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