| Autor: |
Nataša, Mehle, Nina, Prezelj, Matjaž, Hren, Jana, Boben, Kristina, Gruden, Maja, Ravnikar, Marina, Dermastia |
| Rok vydání: |
2012 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 938 |
| ISSN: |
1940-6029 |
| Popis: |
The real-time PCR detection system for grapevine yellows phytoplasmas described here is composed of two assays for group-specific detection of flavescence dorée (FD) and bois noir (BN) phytoplasmas and a universal phytoplasma assay. It uses hydrolysis minor groove binder probes (TaqMan-MGB). The addition of an assay for amplification of plant DNA co-extracted with phytoplasma DNA provides a further quality control for the DNA extraction and PCR amplification for each sample. The detection system described is reliable, specific, sensitive, and easily applicable to fast, high-throughput diagnosis of grapevine yellows phytoplasmas. In addition to the detection system, an approach for the quantification of phytoplasmas in the sample is described. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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