| Autor: |
R R, Fischer, E M, Lucas, A M, Pereira, I, Roisenberg |
| Rok vydání: |
1996 |
| Předmět: |
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| Zdroj: |
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. 29(12) |
| ISSN: |
0100-879X |
| Popis: |
A simple method for the preparation of rabbit antiserum against human von Willebrand factor (vWF) from commercial lyophilized factor VIII concentrate is described. vWF antigen (vWFAg)-like protein was obtained by gel filtration of the concentrate on Sepharose 4B. A combination of measurements of protein content by absorbance at 280 nm, and of vWFAg by electroimmunoassay using a commercial antibody, provided the data needed to select the Sepharose-filtered fractions with the highest concentrations of vWFAg-like protein. The immunization scheme used induced high antibody titers from the 45th to the 126th day after the first immunization. The resulting antiserum showed a performance similar to that of a commercial preparation in terms of vWFAg determination by electroimmunoassay and two-dimensional crossed-immunoelectrophoresis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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