| Autor: |
Aline, Do Minh, Michelle Yen, Tran, Amine A, Kamen |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2086 |
| ISSN: |
1940-6029 |
| Popis: |
The production of lentiviral vectors (LVs) in human embryonic kidney 293 (HEK293) cells using serum-free medium in a suspension culture for the transduction of chimeric antigen receptor T-cells (CAR-T) can be achieved by different methods. This chapter describes LV production by transient transfection, induction of stable packaging cell lines, and induction of stable producer cell lines. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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