Inhibition of murine cytotoxic T lymphocyte activity by a synthetic retroviral peptide and abrogation of this activity by IL
| Autor: | M, Ogasawara, S, Haraguchi, G J, Cianciolo, M, Mitani, R A, Good, N K, Day |
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| Rok vydání: | 1990 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Time Factors Retroviridae Proteins Mice Inbred Strains In Vitro Techniques Biological Factors Mice Viral Envelope Proteins Animals Cytokines Intercellular Signaling Peptides and Proteins Interleukin-2 Interleukin-4 Peptides Cells Cultured T-Lymphocytes Cytotoxic |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 145(2) |
| ISSN: | 0022-1767 |
| Popis: | A synthetic 17 amino acid peptide (CKS-17) homologous to a highly conserved region of human and animal retroviral transmembrane proteins was investigated for its influence on the generation of murine alloantigen-specific CTL activity in vitro. CKS-17 coupled to a carrier protein, BSA or human serum albumin, inhibited the generation of anti-allo CTL in a dose-dependent manner. Controls consisting of BSA and human serum albumin, which had undergone the coupling procedure or neurotensin, an unrelated peptide, coupled to BSA in an identical manner as CKS-17 showed no such inhibitory action. The suppression was not restricted to the Ag specificity of the CTL activity. CKS-17 exerted inhibitory effects on the early afferent phase of CTL induction. Kinetic studies showed that the suppressive activity occurred when CKS-17 was introduced to the immunologically stimulating culture concomitant with or up to 48 h after initiation of culture. Analysis of the frequency of CTL precursor cells using limiting-dilution assays revealed that CKS-17 did act to reduce the number of precursor cells. Abrogation of the inhibition of CTL activity was observed when IL-2 was introduced to the culture together with the stimulator cells. Other lymphokines, such as IL-4, exerted a similar influence to counteract this suppression. |
| Databáze: | OpenAIRE |
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