Characterization and purification of esterase from Cellulomonas fimi DB19 isolated from Zanthoxylum armatum with its possible role in diesel biodegradation

Autor:	Dina, Barman, Mamtaj S, Dkhar
Rok vydání:	2022
Předmět:	Zanthoxylum Enzyme Stability Esterases Temperature Hydrogen Peroxide Hydrogen-Ion Concentration Azo Compounds Cellulomonas
Zdroj:	Archives of microbiology. 204(9)
ISSN:	1432-072X
Popis:	Endophytic bacteria inhabit all or part of their life cycle within the tissues of healthy plants, without causing any apparent symptoms of disease. They are treasure trove of several hydrolytic enzymes with distinct characteristics. Esterase is one of such enzymes and this study aims to characterize esterase produced by endophytic actinobacteria Cellulomonas fimi DB19 isolated from Zanthoxylum armatum with its capacity to degrade diesel oil. The enzyme was purified with purification fold 8.22 and specific activity 124.72 U/mg with 16.43% recovery. The purified enzyme showed a single protein band on SDS-PAGE having molecular mass of approximately 39 kDa. The K
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::57c338a6d201b1e489947eedd1840153 https://pubmed.ncbi.nlm.nih.gov/36030426 Zobrazit plný text záznamu Full text from SpringerLink