Phosphorylated Groucho delays differentiation in the follicle stem cell lineage by providing a molecular memory of EGFR signaling in the niche
| Autor: | Johnston, Michael J., Bar-Cohen, Shaked, Paroush, Ze'ev, Nystul, Todd G. |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Homeodomain Proteins
Receptors Notch Stem Cells Cell Differentiation Epithelial Cells Nerve Tissue Proteins Stem Cells and Regeneration ErbB Receptors Repressor Proteins Drosophila melanogaster Ovarian Follicle Basic Helix-Loop-Helix Transcription Factors Animals Drosophila Proteins lipids (amino acids peptides and proteins) Female RNA Interference Phosphorylation RNA Small Interfering Receptors Invertebrate Peptide Signal Transduction Transcription Factors |
| Popis: | In the epithelial follicle stem cells (FSCs) of the Drosophila ovary, Epidermal Growth Factor Receptor (EGFR) signaling promotes self-renewal, whereas Notch signaling promotes differentiation of the prefollicle cell (pFC) daughters. We have identified two proteins, Six4 and Groucho (Gro), that link the activity of these two pathways to regulate the earliest cell fate decision in the FSC lineage. Our data indicate that Six4 and Gro promote differentiation towards the polar cell fate by promoting Notch pathway activity. This activity of Gro is antagonized by EGFR signaling, which inhibits Gro-dependent repression via p-ERK mediated phosphorylation. We have found that the phosphorylated form of Gro persists in newly formed pFCs, which may delay differentiation and provide these cells with a temporary memory of the EGFR signal. Collectively, these findings demonstrate that phosphorylated Gro labels a transition state in the FSC lineage and describe the interplay between Notch and EGFR signaling that governs the differentiation processes during this period. |
| Databáze: | OpenAIRE |
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