DNA fingerprinting of septicemic and localized Burkholderia pseudomallei isolates from Malaysian patients

Autor: M N, Azura, A, Norazah, A G M, Kamel, S Ahmad, Zorin
Rok vydání: 2011
Předmět:
Zdroj: The Southeast Asian journal of tropical medicine and public health. 42(1)
ISSN: 0125-1562
Popis: We have analysed DNA fingerprinting patterns by pulsed-field gel electrophoresis (PFGE) of 52 unrelated Burkholderia pseudomallei strains isolated from septicemic and localized infections from Malaysian subjects. A total of 38 PFGE types were observed among 36 septicemic and 16 localized strains with no predominant pattern. Type 25 was seen in 2 epidemiologically related strains, suggesting human to human transmission. Twelve PFGE types were shared among 26 strains (21 septicemic and 5 localized) showing close genetic relatedness with coefficient of similarity of 0.81 to 1.0. The other 26 strains (15 septicemic and 11 localized) were unrelated as shown by the similarity coefficient of0.8. This study showed that our B. pseudomallei strains in Malaysia were mainly heterogenous with no predominant type both in septicemic or localized strains.
Databáze: OpenAIRE