Mitochondrial membrane potential (delta psi(mt)) in the coordination of p53-independent proliferation and apoptosis pathways in human colonic carcinoma cells
| Autor: | B G, Heerdt, M A, Houston, G M, Anthony, L H, Augenlicht |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Cyclin-Dependent Kinase Inhibitor p21
Uncoupling Agents Apoptosis Cytochrome c Group Intracellular Membranes Flow Cytometry Membrane Potentials Mitochondria Butyrates Cyclins Rotenone Colonic Neoplasms Tumor Cells Cultured Butyric Acid Humans Interphase Cell Division Thenoyltrifluoroacetone Cell Aggregation |
| Zdroj: | Cancer research. 58(13) |
| ISSN: | 0008-5472 |
| Popis: | We have previously defined depressed mitochondrial function as a determinant in colon cancer risk and progression and established that metabolism of butyrate, a short-chain fatty acid generated during the fermentation of fiber by endogenous intestinal bacteria, induces mitochondrial function-dependent growth arrest and apoptosis of colonic carcinoma cells in vitro. Here, we dissect the relationships among mitochondrial function, growth arrest, and apoptosis, reporting that initiation and maintenance of butyrate-mediated p53-independent p21WAF1/Cip1 induction and subsequent G0/G1 arrest require an intact mitochondrial membrane potential (delta psi(mt)) and that the process of dissipation of the delta psi(mt) is then essential for initiation of a butyrate-induced apoptotic cascade. Thus, we hypothesize that mitochondria play a pivotal role in coordinating proliferation and apoptosis pathways, a coordination that must be tightly regulated in rapidly renewing tissues, such as the colonic mucosa. |
| Databáze: | OpenAIRE |
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