| Autor: |
Mohamad, Moghadam, Mehran, Karimi, Seyed Javad, Dehghani, Javad, Dehbozorgian, Somaye, Montazeri, Elham, Javanmardi, Rahimeh, Asadzade, Azizollah, Amiri, Zahra, Saghatoleslam, Fatemosadat, Sotodegan, Nazila, Morshedi, Jaber, Imanifard, Abdolreza, Afrasiabi |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Prenatal diagnosis. 35(12) |
| ISSN: |
1097-0223 |
| Popis: |
The aim of this study was to evaluate the effectiveness of prenatal diagnosis (PND) for the prevention of thalassemia in Southern Iran.From 2004 to 2012 1346 couples with β-thalassemia minor were referred to our center. Mutation analyses utilized different methods including polymerase chain reaction-based technique of amplification refractory mutation system (ARMS), Restriction Fragment Length Polymorphism Analysis of PCR-Amplified Fragments (PCR-RFLP) and Gel Electrophoresis and direct sequencing. Haplotype analysis of the β-globin gene cluster was done routinely using the PCR-RFLP technique.Of the 1346 couples, 884 (66%) requested PND. They had a total of 985 pregnancies (954 singleton and 31 twin pregnancies): the 1016 fetuses underwent chorionic villus sampling (CVS). Thalassemia major was diagnosed in 266 cases (26.2%), and termination of pregnancy was requested by the parents in 264 of them (99%). Thalassemia trait was detected in 499 (49.1%) and 251 cases (24.7%) showed no β-thalassemia mutations. There were three misdiagnoses (0.4%) (affected children diagnosed as carriers at PND). A unique pattern of thalassemia mutations was present in the study population, with IVS II-I (G→A), C36-37(-T), IVS I-5(GC), -25bpdel (252-276), IVS I-110(GA) and C44 (-C) being present in 62% of cases.The pattern of distribution of thalassemia mutations differs among ethnic groups within the same country. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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