Short-term effect of epidermal growth factor on glucose uptake in endoscopic biopsies
| Autor: | Dalal, Tonb, Raj, Mehta, Hugh, Wang, John, Tung, Devendra I, Mehta |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Membrane Glycoproteins Adolescent Epidermal Growth Factor Monosaccharide Transport Proteins Biopsy Infant Methylglucosides Water-Electrolyte Balance Endoscopy Gastrointestinal Enterocytes Glucose Sodium-Glucose Transporter 1 Intestinal Absorption Child Preschool Culture Techniques Humans Female Intestinal Mucosa Child |
| Zdroj: | Digestive diseases and sciences. 48(8) |
| ISSN: | 0163-2116 |
| Popis: | Epidermal growth factor (EGF) up-regulation of glucose absorption via increased Na+/glucose co-transporter (SGLT-1) activity has previously been described in rabbit jejunal brush-border membrane and in differentiated Caco-2 cells. The goal of the present study was to assess the in vitro effect of EGF (200 ng/ml) on glucose uptake in human mucosal specimens, and we describe a simple procedure that uses endoscopic biopsies for short-term gludose uptake measurements. Uptake values for the EGF-treated biopsies ranged from 2.7 to 29.0, with a mean uptake of 10.65, while uptake values for the untreated biopsies ranged from 0.9 to 17.5, with a mean uptake of 7.99 (P0.05, paired t test). This early effect of EGF on human enterocytes may have important therapeutic implications. A role in increasing the rate of internal rehydration is suggested. |
| Databáze: | OpenAIRE |
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