Strategic aromatic residues in the catalytic cleft of the xyloglucanase MtXgh74 modifying thermostability, mode of enzyme action, and viscosity reduction ability

Autor:	Oksana V, Berezina, Sergey V, Rykov, Angelina K, Polyakova, Marine E, Bozdaganyan, Anna V, Sidochenko, Melanie, Baudrexl, Wolfgang H, Schwarz, Vladimir V, Zverlov, Sergey V, Yarotsky
Rok vydání:	2020
Předmět:	Glycoside Hydrolases Viscosity Sordariales Xylans Substrate Specificity
Zdroj:	Applied microbiology and biotechnology. 105(4)
ISSN:	1432-0614
Popis:	The thermostable endo-processive xyloglucanase MtXgh74 from Myceliophthora thermophila was used to study the influence of aromatic amino acids in the catalytic cleft on the mode of action and the ability of enzyme to reduce xyloglucan viscosity. The enzyme derivative Mut I with mutations W64A/W67A in the "negative" subsites of the catalytic cleft resulted in a 5.5-fold increase of the K
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::56c3d76d4dc02d44d941e8fe0e5ec151 https://pubmed.ncbi.nlm.nih.gov/33521846 Zobrazit plný text záznamu Full text from SpringerLink