[Mongolian medicine cha gan beng ga regulated activity of biomarker PGC-1α]
| Autor: | Han-Qing, Li, Jia-Yin, Xu, Lu, Yu, Ji-Le, Xin, Ji-Wen, Wei |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
Medicine Mongolian Traditional Aconitum Lung Neoplasms Time Factors Dose-Response Relationship Drug Plant Extracts Reverse Transcriptase Polymerase Chain Reaction Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha Gene Expression Regulation Neoplastic Mice Inbred C57BL Carcinoma Non-Small-Cell Lung Cell Line Tumor Biomarkers Tumor Animals Humans Lung Transcription Factors |
| Zdroj: | Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. 39(17) |
| ISSN: | 1001-5302 |
| Popis: | To investigate the regulation of Cha Gan Beng Ga on the activity of biomarker PGC-1α in vivo and in vitro, and lay the foundation for studying the efficacy result of Cha Gan Beng Ga on xenograft tumor model and extracting active constituents.(1) The coarse powder of Cha Gan Beng Ga was extracted with 70% ethanol solution through heating and refluxing, and finally was used to freeze dry powder. (2) 50 mg x kg(-1) of freeze-dried power was orally administrated to KM and C57BL/6J mice once daily, lasting for 5 consecutive days; different concentrations of extracted materials was given to non-small cell lung cells A549. (3) The expression level of PGC-1α mRNA was quantitatively determined in lung tissue of mice and non-small cell lung cells A549.The expression levels of PGC-1α in lung tissue of different mice strains had an increasing tendency. Furthermore, the expression levels of PGC-1α in non-small cell lung cells A549 also had an increasing tendency, showing dose and time-dependent relationships.Mongolian Medicine Cha Gan Beng Ga could induce the over-expression of PGC-1α mRNA in lung tissue of mice and in non-small cell lung cells A549. The present results will lay foundation for studying the efficacy result of antitumor and active constitutes in future. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|