Expression profiling identifies a novel alpha-methylacyl-CoA racemase exon with fumarate hydratase homology
| Autor: | Grace L, Shen-Ong, Yun, Feng, Dean A, Troyer |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Male
Prostatic Diseases DNA Complementary Base Sequence Gene Expression Profiling Molecular Sequence Data Racemases and Epimerases Prostatic Neoplasms Sequence Homology Adenocarcinoma Fumarate Hydratase Mitochondria Neoplasm Proteins Protein Structure Tertiary Alternative Splicing Peroxisomes Humans Amino Acid Sequence RNA Messenger Sequence Alignment Oligonucleotide Array Sequence Analysis |
| Zdroj: | Cancer research. 63(12) |
| ISSN: | 0008-5472 |
| Popis: | Human alpha-methylacyl-CoA racemase (AMACR) was overexpressed in prostate cancer compared with nonmalignant tissues. The Gene Logic Inc. BioExpress database containing Affymetrix U133 GeneChip expression profiles of 4400 human normal, benign, diseased, and tumor samples from60 tissue types was examined to determine the specificity of AMACR mRNA expression. One particular AMACR probeset was derived from an alternatively spliced exon with 88% identity to a 521-bp sequence that spans four exons of the fumarate hydratase. The predicted protein sequence revealed a novel GLGELIL peptide shared by both proteins. Whether the mitochondrial and peroxisomal AMACR described previously are distinct products from alternatively spliced transcripts remains to be determined. The determination of the cellular location and function of the altered AMACR will be critical in the elucidation of the role of AMACR in prostate cancer diagnosis and pathogenesis. |
| Databáze: | OpenAIRE |
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