Phagocytosis of latex beads is defective in cultured human retinal pigment epithelial cells with persistent rubella virus infection
| Autor: | Williams, L. L., Lew, H. M., Shannon, B. T., Singley, C. T., Davidorf, F. H., Jin, R., Wolinsky, J. S. |
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| Jazyk: | angličtina |
| Rok vydání: | 1993 |
| Předmět: |
Base Sequence
Latex Molecular Sequence Data Virion Fluorescent Antibody Technique Genome Viral Flow Cytometry eye diseases Actins Microspheres Microscopy Electron Phagocytosis Reference Values Microscopy Electron Scanning Animals Humans sense organs Oligonucleotide Probes Pigment Epithelium of Eye Vero Cells Cells Cultured Rubella Research Article |
| Popis: | Phagocytosis, a secondary function of retinal pigment epithelial (RPE) cells essential to sight, was significantly decreased, when measured with latex beads, during persistent rubella virus (RV) infection of human cultured RPE cells. A target for RV in vivo, RPE cells infected with RV (RPE/RV) ingested fewer fluorescent microspheres (26%) than did uninfected RPE cells (68%) (P < 0.001), as measured by flow cytometry. In RPE/RV cells, with characteristic RPE monolayer appearance and normal growth during subculturing over 6 months, persistent RV infection was shown by specific RV antigen immunofluorescence, by the presence of the RV genome in RPE/RV cell messenger RNA, and by recovery of cell-free RV after cocultivation with Vero cells. The adhesion of latex beads to apical cell surfaces of RPE/RV and uninfected RPE cells appeared similar, as imaged by scanning electron microscopy. Cytoskeletal actin, a component of phagocytosis in RPE, appeared altered in 60 to 75% of RPE/RV cells by antiactin immunofluorescence staining, as previously described in other RV-infected cells, but its role in the disturbed phagocytosis of latex beads was not determined. Persistently RV-infected human RPE is an additional example of RV-associated secondary cellular dysfunction in the absence of cytopathic effects. |
| Databáze: | OpenAIRE |
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