Characterization of Achromobacter Species in Cystic Fibrosis Patients: Comparison of blaOXA-114 PCR Amplification, Multilocus Sequence Typing, and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Autor:	Rodrigues, Elenice R. A., Ferreira, Alex G., Leão, Robson S., Leite, Cassiana C. F., Carvalho-Assef, Ana Paula, Albano, Rodolpho M., Marques, Elizabeth A.
Jazyk:	angličtina
Rok vydání:	2015
Předmět:	Cystic Fibrosis Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Humans Bacteriology Achromobacter Gram-Negative Bacterial Infections Polymerase Chain Reaction beta-Lactamases Multilocus Sequence Typing
Popis:	Molecular methodologies were used to identify 28 Achromobacter spp. from patients with cystic fibrosis (CF). Multilocus sequence typing (MLST) identified 17 Achromobacter xylosoxidans isolates (all bla(OXA-114) positive), nine Achromobacter ruhlandii isolates (all bla(OXA-114) positive), one Achromobacter dolens isolate, and one Achromobacter insuavis isolate. All less common species were misidentified as A. xylosoxidans by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Chronic colonization by clonally related A. ruhlandii isolates was demonstrated.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::5645efe85e15b67d08cab73c1c5dd6d9 https://europepmc.org/articles/PMC4652089/ Zobrazit plný text záznamu