Variants of a Leishmania surface antigen derived from a multigenic family
| Autor: | P J, Murray, T W, Spithill |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Leishmania
Base Sequence Molecular Sequence Data Protozoan Proteins Nucleic Acid Hybridization Antigens Protozoan DNA Protozoan Blotting Northern Electrophoresis Gel Pulsed-Field Blotting Southern Multigene Family Antigens Surface Animals Amino Acid Sequence Sequence Alignment RNA Protozoan Repetitive Sequences Nucleic Acid |
| Zdroj: | The Journal of biological chemistry. 266(36) |
| ISSN: | 0021-9258 |
| Popis: | The promastigote surface antigen-2 (PSA-2) complex comprises a group of immunogenic surface antigens linked to the surface of the Leishmania major promastigote with glycosylphosphatidylinositol anchors. The L. major genome contains at least 14 PSA-2 genes on a 950-kilobase chromosome and comprising approximately 20% of the length of this chromosome. The sequence of three independent, but incomplete, PSA-2 cDNAs and one genomic fragment encoding a complete PSA-2 coding sequence were compared. PSA-2 genes encode polypeptides exhibiting 22-25aa tandem repeat elements, threonine-rich segments which vary between genes, a conserved COOH-terminal cysteine-rich region, and a conserved GPI anchor signal sequence. PSA-2 genes appear to be transcribed in a complex manner with multiple RNAs. The complex genomic organization of PSA-2 genes is present in other members of the genus suggesting that PSA-2 function is important for the biology of Leishmania. |
| Databáze: | OpenAIRE |
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