Leptin Mediates
| Autor: | Glaucia, Souza-Almeida, Heloisa, D'Avila, Patricia E, Almeida, Tatiana, Luna-Gomes, Sally, Liechocki, Barbara, Walzog, Ingrid, Hepper, Hugo Caire, Castro-Faria-Neto, Patricia T, Bozza, Christianne, Bandeira-Melo, Clarissa M, Maya-Monteiro |
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| Rok vydání: | 2017 |
| Předmět: |
Leptin
Male Mice Knockout Mice Inbred C3H Arachidonate 5-Lipoxygenase Neutrophils Tumor Necrosis Factor-alpha Chemokine CXCL1 digestive oral and skin physiology Immunology neutrophil leukotriene B4 CXCL1 Mice Inbred C57BL Phosphatidylinositol 3-Kinases Neutrophil Infiltration Cell Movement Receptors Tumor Necrosis Factor Type I inflammation Macrophages Peritoneal Animals hormones hormone substitutes and hormone antagonists Chemokine CCL3 Original Research |
| Zdroj: | Frontiers in Immunology |
| ISSN: | 1664-3224 |
| Popis: | Leptin directly activates macrophages and lymphocytes, but the role of leptin in neutrophil activation and migration is still controversial. Here, we investigate the in vivo mechanisms of neutrophil migration induced by leptin. The intraperitoneal injection of leptin (1 mg/kg) induces a time- and concentration-dependent neutrophil influx. We did not observe the enhancement of lipid bodies/droplets in neutrophils, after leptin treatment, as we had observed previously in peritoneal macrophages. The participation of leukotriene B4 (LTB4) in neutrophil recruitment triggered by leptin was investigated using different strategies. Leptin-induced neutrophil recruitment occurs both in the absence of 5-lipoxygenase activity in 5-lipoxygenase (5-LO)−/− mice and after the administration of either 5-LO inhibitor (Zileuton) or the LTB4 receptor antagonist (U-75302). Moreover, no direct induction of LTB4 by leptin could be observed. Neutrophil influx could not be prevented by the mammalian target of rapamycin (mTOR) inhibitor, rapamycin, contrasting with the leptin-induced signaling for lipid body formation in macrophage that is mTOR-dependent. Leptin administration led to tumor necrosis factor-alpha (TNFα) production by the peritoneal cells both in vivo and in vitro. In addition, neutrophil recruitment was inhibited in tumor necrosis factor receptor 1 (TNFR1−/−) mice, indicating a role for TNF in leptin-induced neutrophil recruitment to the peritoneal cavity. Leptin-induced neutrophil influx was PI3Kγ-dependent, as it was absent in PI3Kγ−/− mice. Accordingly, leptin induced the peritoneal cells to produce CXCL1, both in vivo and in vitro, and the neutrophil influx was ablated after using an antibody against CXCL1. Our results establish TNFα/TNFR1- and CXCL1-dependent signaling as important pathways for leptin-induced neutrophil migration in vivo. |
| Databáze: | OpenAIRE |
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