| Popis: |
To determine the expression level of linc-223 and miR-125a in the patients with adult acute leukemia (AL) and explore the relationship between the expression level and the occurrence, development, prognosis of leukemia.Bone marrow samples of 93 patients with AL treated in our hospital from January 2017 to September 2017 were enrolled, including 21 cases of acute lymphoblastic leukemia (ALL) and 72 cases of acute non-lymphocytic leukemia (ANLL). At the same time, bone marrow samples from 20 cases of non-malignant hematopathy patients in the same period were enrolled as control group. Real-time quantitative PCR (qRT-PCR) was used to test the expression level of linc-223 and miR-125a in bone marrow of 93 AL patients and the relationship between the level and the occurrence, development, prognosis of leukemia was analyzed.There was no significant difference in the expression of linc-223 between AL patients (ANLL and ALL) and control group (P0.05). Moreover, there was no significant correlation between linc-223 and PML-RARα gene or the remission rate of patients after treatment. The expression of miR-125a in ANLL patients was significantly lower than those in the control group (P0.01), but there was no significant difference between the initial treatment, recurrence and remission group (P0.05), and also for ALL and control group (P0.05). In the newly treatment ANLL patients, the expression level of miR-125a showed negatively correlated with LDH level and the ratio of immature cells (r=-0.454, r=-0.400), but not with sex, degree of risk, peripheral blood leukocyte count, platelet count, hemoglobin content, WT1, CRP, etc. (P0.05). There was a positive correlation between linc-223 and miR-125a in ANLL patients (r=0.296).No abnormal expression of linc-223 was found in the bone marrow of AL patients, but miR-125a expression shows a low level and positively correlate with the expression level of linc-223 in ANLL, which is helpful for the diagnosis.linc-223、miR-125a在急性白血病患者中的表达及其临床意义.明确linc-223、miR-125a在成人急性白血病（AL）患者中的表达水平，并探索其表达水平与白血病的发生、发展及预后之间的关系.选取2017年1月至 2017年9月入住本院93例AL患者的骨髓标本，其中急性淋巴细胞白血病（ALL）21例，急性非淋巴细胞白血病（ANLL）72例。同时收集同期住院的20例非恶性血液病患者的骨髓标本作为对照组。采用实时荧光定量PCR检测93例AL患者骨髓中linc-223与miR-125a的表达水平并分析其与白血病的发生、发展及预后的关系.在AL(ANLL和ALL）患者和对照组中，linc-223的表达无统计学差异（P0.05），并且其与PML-RARα基因和患者治疗后的缓解率无相关性。miR-125a在ANLL患者中的表达量低于对照组 (P0.01)，但在初治组、复发组与缓解组之间的表达差异并无统计学意义（P0.05），并且在ALL和对照组中的表达差异也无统计学意义(P0.05)。在ANLL初治组患者中，miR-125a的表达水平与LDH水平、幼稚细胞比例呈负相关（r=-0.454，r=-0.400），与患者性别、危险度分组、外周血白细胞数、血小板计数、血红蛋白含量、WT1、CRP等均未发现存在相关性（P0.05）。在linc-223与 miR-125a的相关性分析中， linc-223与 miR-125a表达水平在ANLL患者中存在正相关（r=0.296）.在AL患者中，未发现linc-223表达存在异常，但是miR-125a在ANLL中存在低表达，并且与linc-223间的表达水平存在正相关，这有助于对ANLL的诊断. |
