CYP4F2 1347 GAGGCX 12970 CG polymorphisms: frequency in north Indianstheir effect on dosing of acenocoumarol oral anticoagulant
| Autor: | Saurabh Singh, Rathore, Surendra Kumar, Agarwal, Shantanu, Pande, Sushil Kumar, Singh, Tulika, Mittal, Balraj, Mittal |
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| Rok vydání: | 2014 |
| Předmět: |
Heart Valve Prosthesis Implantation
CYP2C9 Allele frequency Acenocoumarol anticoagulant Anticoagulants India north Indians Polymerase Chain Reaction Polymorphism Single Nucleotide GGCX Biomarkers Pharmacological polymorphism Logistic Models Carbon-Carbon Ligases Cytochrome P-450 Enzyme System Gene Frequency Pharmacogenetics Humans Original Article Cytochrome P450 Family 4 Polymorphism Restriction Fragment Length |
| Zdroj: | The Indian Journal of Medical Research |
| ISSN: | 0971-5916 |
| Popis: | Background & objectives: CYP4F2 and γ-glutamyl carboxylase (GGCX) have small but significant roles in the maintenance dose of coumarinic oral anticoagulants (COAs). CYP4F2 1347 G>A and GGCX 12970 C>G polymorphisms have been used in the pharmacogenetic dosing algorithms of warfarin for Caucasians and Chinese populations. India has a large population with multiple ethnic groups but there are no reports about the frequencies of these polymorphisms in north Indians. In the present study, we aimed to find out the allelic frequencies of CYP4F2 1347 G>A and GGCX 12970 C>G polymorphisms in a north Indian population and relate these to daily maintenance drug dose requirements of COA. Methods: CYP4F2 1347 G>A and GGCX 12970 C>G polymorphisms were genotyped by polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) protocols and Taqman SNP discrimination assays in healthy volunteers (n=102) and patients (n=225) receiving acenocoumarol, an oral anticoagulant, after cardiac valve replacement surgery. Results: In healthy volunteers, the allele frequencies for CYP4F2 1347 G>A and GGCX 12970 C>G were 43.14 and 1.43 per cent, respectively. No significant differences in mean weight normalized doses of acenocoumarol were found for these CYP4F2 and GGCX genotypes. Binary logistic regression analysis revealed no significant association of any of the genotypes or alleles with the dosing phenotypes for both the SNPs. Interpretation & conclusions: We report distinct frequencies of CYP4F2 1347 G>A and GGCX 12970 C>G polymorphisms in north Indians but these polymorphisms did not have significant bearing on maintenance dose of acenocoumarol oral anticoagulant in cardiac valve replacement patients. |
| Databáze: | OpenAIRE |
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