Molecular Genotyping of Microbes by Multilocus PCR and Mass Spectrometry: A New Tool for Hospital Infection Control and Public Health Surveillance
| Autor: | Ecker, David J., Massire, Christian, Blyn, Lawrence B., Hofstadler, Steven A., Hannis, James C., Eshoo, Mark W., Hall, Thomas A., Sampath, Rangarajan |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Acinetobacter baumannii
DNA Bacterial Genetics Microbial Ibis T5000 Cross Infection Molecular Epidemiology Spectrometry Mass Electrospray Ionization Genotype Streptococcus pyogenes Molecular genotyping Polymerase Chain Reaction Polymorphism Single Nucleotide Article Disease Outbreaks base composition Genes Bacterial Influenza A virus Population Surveillance Streptococcal Infections DNA Viral Humans PCR/ESI-MS mass spectrometry Acinetobacter Infections |
| Zdroj: | Molecular Epidemiology of Microorganisms |
| Popis: | We describe a new technology for the molecular genotyping of microbes using a platform known commercially as the Ibis T5000. The technology couples multilocus polymerase chain reaction (PCR) to electrospray ionization/mass spectrometry (PCR/ESI-MS) and was developed to provide rapid, high-throughput, and precise digital analysis of either isolated colonies or original patient specimens on a platform suitable for use in hospital or reference diagnostic laboratories or public health settings. The PCR/ESI-MS method measures digital molecular signatures from microbes, enabling real-time epidemiological surveillance and outbreak investigation. This technology will facilitate understanding of the pathways by which infectious organisms spread and will enable appropriate interventions on a time frame not previously achievable. |
| Databáze: | OpenAIRE |
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