| Autor: |
V M, Zaĭdes, V R, Farashian, Iu A, Boshchenko, V M, Zhdanov |
| Rok vydání: |
1981 |
| Předmět: |
|
| Zdroj: |
Biokhimiia (Moscow, Russia). 46(12) |
| ISSN: |
0320-9725 |
| Popis: |
The monolayer cultures of chicken embryonic cells were infected with influenza virus and 6 hrs after the infection the relative concentrations of viral mRNAs and the relative rates of virus-specific protein synthesis were determined. For this purpose the infected cells were incubated under conditions of continuous labelling of RNAs by the radioactive precursor; the mRNA fraction was obtained by hybridization of total RNA of infected cells with non-labelled RNA of viral particles and the individual mRNAs were fractionated by polyacrylamide gel electrophoresis of RNA duplexes. The proteins from infected cells were labelled by [14C] amino acids for a short time and the individual virus-specific proteins were separated electrophoretically. There was no correlation between the rates of virus-specific protein synthesis and the relative concentrations of corresponding mRNAs. This indicates the regulation of virus-specific protein synthesis at the post-translational level. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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