Effects of serum on (-)-gossypol-suppressed growth in human prostate cancer cells
| Autor: | Yi-Wen, Huang, Li-Shu, Wang, Hsiang-Lin, Chang, Weiping, Ye, Yasuro, Sugimoto, Michael K, Dowd, Peter J, Wan, Young C, Lin |
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| Rok vydání: | 2006 |
| Předmět: |
Cyclin-Dependent Kinase Inhibitor p21
Male Serum Neoplasms Hormone-Dependent Cell Survival Reverse Transcriptase Polymerase Chain Reaction Blotting Western Gossypol Cyclin-Dependent Kinase 4 Prostatic Neoplasms Dextrans Serum Albumin Bovine Cyclin-Dependent Kinase 6 Retinoblastoma Protein Transforming Growth Factor beta1 Charcoal Tumor Cells Cultured Animals Humans Cattle Cyclin D1 RNA Messenger RNA Neoplasm |
| Zdroj: | Anticancer research. 26(5A) |
| ISSN: | 0250-7005 |
| Popis: | Gossypol, a natural polyphenolic compound present in cottonseeds, possesses antiproliferative and pro-apoptotic effects in in vivo and in vitro models. There are two enantiomers, (+)-gossypol and (-)-gossypol, the latter being a more potent inhibitor of cancer cell growth. Here, the effect of bovine serum albumin (BSA) and dextran-coated charcoal-treated fetal bovine serum (DCC-FBS)-containing medium on the ability of (-)-gossypol to inhibit the growth of human prostate cancer cells was studied.BSA- and DCC-FBS-supplemented medium were used to examine the influence of serum proteins on the antiproliferative effects of (-)-gossypol in DU-145 cells, a human prostate cancer cell line. The viability of the DU-145 cells was determined by CellTiter 96 Aqueous assay. The expressions of mRNA and protein for the cell cycle regulators, cyclin-D1, Rb, CDK, p21 and TGF-beta, were determined by RT-PCR and Western blot analyses, respectively.(-)-Gossypol caused growth suppression of the DU-145 cells. In comparison with BSA-supplemented medium, DCC-FBS blocked the antiproliferative effects of (-)-gossypol at 1 and 2.5 microM, but not at 5 microM. Furthermore, (-)-gossypol treatment down-regulated cyclin-D1, Rb, CDK4 and CDK6, and up-regulated p21 and TGF-beta1 at the mRNA and/or protein levels.The data suggested that (-)-gossypol-suppressed prostate cancer cell growth may be influenced through cell cycle regulators, which may lead to better prognosis. We further speculate that (-)-gossypol might serve as a chemotherapeutic agent for human prostate cancer patients. |
| Databáze: | OpenAIRE |
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