| Autor: |
Marin, Barisic, Ricardo, Silva e Sousa, Suvranta K, Tripathy, Maria M, Magiera, Anatoly V, Zaytsev, Ana L, Pereira, Carsten, Janke, Ekaterina L, Grishchuk, Helder, Maiato |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Science (New York, N.Y.). 348(6236) |
| ISSN: |
1095-9203 |
| Popis: |
Before chromosomes segregate into daughter cells, they align at the mitotic spindle equator, a process known as chromosome congression. Centromere-associated protein E (CENP-E)/Kinesin-7 is a microtubule plus-end-directed kinetochore motor required for congression of pole-proximal chromosomes. Because the plus-ends of many astral microtubules in the spindle point to the cell cortex, it remains unknown how CENP-E guides pole-proximal chromosomes specifically toward the equator. We found that congression of pole-proximal chromosomes depended on specific posttranslational detyrosination of spindle microtubules that point to the equator. In vitro reconstitution experiments demonstrated that CENP-E-dependent transport was strongly enhanced on detyrosinated microtubules. Blocking tubulin tyrosination in cells caused ubiquitous detyrosination of spindle microtubules, and CENP-E transported chromosomes away from spindle poles in random directions. Thus, CENP-E-driven chromosome congression is guided by microtubule detyrosination. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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