Genomic organization of the translocations (8;14) and (14;18) in a new lymphoma cell line
| Autor: | A C, Lambrechts, L C, Dorssers, P E, Hupkes, R, van Gurp, R, Baumgarten, A, Hagemeijer, M B, van 't Veer, I P, Touw |
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| Rok vydání: | 1994 |
| Předmět: |
Chromosomes
Human Pair 14 Genome Human Genes myc Gene Expression Polymerase Chain Reaction Translocation Genetic Proto-Oncogene Proteins c-bcl-2 Bone Marrow Karyotyping Proto-Oncogene Proteins Tumor Cells Cultured Humans Female RNA Messenger Chromosomes Human Pair 18 Lymphoma Large-Cell Immunoblastic Aged Chromosomes Human Pair 8 |
| Zdroj: | Leukemia. 8(7) |
| ISSN: | 0887-6924 |
| Popis: | We generated a new lymphoma cell line carrying the translocations (8;14) and (14;18) and studied the genomic organization and expression of the BCL-2 and MYC genes. Polymerase chain reaction (PCR) and Southern analysis showed that the breakpoints of t(14;18) were located in the major breakpoint region (mbr) of the BCL-2 gene and just 5' of JH6 in the IgH locus. The breakpoints of the t(8;14) were located upstream of exon 2 in the non-coding region of the MYC gene and near the switch region of the IgH locus. Both IgH loci were involved in chromosomal translocations resulting in the absence of a functional B-cell receptor. Normal BCL-2 and truncated MYC transcripts were detected in these cells. The BCL-2 protein was expressed. |
| Databáze: | OpenAIRE |
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