| Autor: |
Marie L, Davies, Emma J, Taylor, Caroline, Gordon, Stephen P, Young, Ken, Welsh, Mike, Bunce, B Paul, Wordsworth, Brian, Davidson, Simon J, Bowman |
| Rok vydání: |
2002 |
| Předmět: |
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| Zdroj: |
Arthritis and rheumatism. 46(1) |
| ISSN: |
0004-3591 |
| Popis: |
To identify T cell epitopes of the human La autoantigen involved in the generation of anti-Ro/La autoantibodies.Molecular techniques were used for HLA typing of 219 white patients with systemic lupus erythematosus and 125 white patients with primary Sjögren's syndrome. Anti-Ro/La antibody levels were measured by enzyme-linked immunosorbent assay. Peripheral blood mononuclear cell responses to an overlapping series of synthetic 15-mer peptides spanning the entire La sequence were examined in pools or individually in conventional 7-day proliferation assays.HLA typing confirmed that the HLA-DR3/DQ2 haplotype is closely associated with the occurrence of anti-Ro/La antibodies, and that the frequency of HLA-DR1 and DR4 haplotypes is reduced among antibody-positive patients. We identified 3 regions of the La sequence likely to contain T cell epitopes and 1 peptide, La 49-63, that generated a low-level but clear-cut T cell proliferative response. The HLA restrictions of these responses mirrored the HLA association data from the cohort study. Among individuals who were HLA-DR3 positive, there was no difference between patients and controls in the proliferative response to the La 49-63 peptide.Our data suggest that these are naive T cell responses, and that the identification of T cell epitopes involved in the generation of anti-Ro/La autoantibodies should focus on alternative candidate antigens. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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