A recombinant protein from Schistosoma mansoni useful for the detection of S. mansoni and Schistosoma haematobium antibodies
| Autor: | K, Hancock, Y B, Mohamed, X, Haichou, J, Noh, E M, Dotson, V C, Tsang |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Plasmodium
Base Sequence Molecular Sequence Data Antibodies Helminth Antibodies Protozoan Enzyme-Linked Immunosorbent Assay Helminth Proteins Schistosoma mansoni Cross Reactions DNA Helminth Macaca mulatta Sensitivity and Specificity Recombinant Proteins Schistosoma japonicum Schistosomiasis mansoni Malaria Schistosomiasis haematobia Species Specificity Antigens Helminth Schistosoma haematobium Animals Humans Cloning Molecular |
| Zdroj: | The Journal of parasitology. 83(4) |
| ISSN: | 0022-3395 |
| Popis: | A recombinant Schistosoma mansoni protein has been identified as a useful antigen for the detection of S. mansoni and Schistosoma haematobium antibodies. The purified recombinant protein, Sm22.3, was assayed using an enzyme-linked immunosorbent assay format against a battery of 491 well defined sera, including S. mansoni, S. haematobium, and Schistosoma japonicum infection sera, normal human sera, sera from 9 other parasitic infections, and sera from 2 additional infections. The sensitivity for detecting S. mansoni and S. haematobium infections with this single recombinant protein is 80.1%. The specificity is 94.8%. However, 15 of the 16 cross-reactive sera are malaria infection sera, and we have data suggesting that these malaria sera are actually recognizing an epitope on the vector-derived 6Xhistidine tag of recombinant Sm22.3. If this is the case, then, the actual specificity of the assay is 99.6%. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|