| Autor: |
N N, Sokolov, A B, Fitsner, N V, Anikeĭcheva, N A, Kovalenko |
| Rok vydání: |
1989 |
| Předmět: |
|
| Zdroj: |
Voprosy meditsinskoi khimii. 35(6) |
| ISSN: |
0042-8809 |
| Popis: |
Electrophoretic analysis of products obtained after hydrolysis of phage lambda DNA by means of restrictase Pae I was carried out after preincubation of DNA or the enzyme with Mg2+ as well as after preincubation of DNA simultaneously with the enzyme and the subsequent addition of the required components into the experimental samples. The analysis showed that Mg2+ were apparently not required for the enzyme-substrate complex formation and caused destabilization of the complex. Restrictase Pae I was in active form when Mg2+ was substituted by Mn2+, Ca2+, Zn2+, Co2+ but not by Cd2+, Cu2+ or Ni2+. Experiments with o-phenanthroline showed that Zn2+ cations are of importance in catalytic activity of restrictase Pae I. Possible functions of Zn2+ in protein-nucleic acids recognition are discussed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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