| Autor: |
E, Mahdy, Y, Pan, N, Wang, P U, Malmström, P, Ekman, U, Bergerheim |
| Rok vydání: |
2002 |
| Předmět: |
|
| Zdroj: |
Anticancer research. 21(5) |
| ISSN: |
0250-7005 |
| Popis: |
Chromosome 8 aberration and c-myc amplification have been suggested as playing important roles in the development of different human cancers. Using fluorescence in situ hybridization (FISH), chromosome 8 polysomy and c-myc amplification can be detected in cells from bladder cancer. We investigated the correlation of chromosome 8 polysomy, c-myc gene alteration and p53 deletion with histopathological parameters. Twenty-four tumors obtained from patients with bladder cancer were analyzed by interphase cytogenetics using FISH with chromosome 8 and 17 centromere probes together with an YAC clone covering the c-myc locus and three cosmid DNA probes covering the p53 locus. Chromosome 8 polysomy was found in 12 tumors. The average copy number of chromosome 8 centromere signals were significantly higher in high grade and stage, cancers. Also the c-myc copy gain and p53 deletion were significantly correlated with grade as well as stage (p0.05, in both cases). Both polysomy 8 and c-myc copy gain were significantly correlated with p53 deletions (p0.01) and DNA ploidy (p0.001). On the contrary there was no significant correlation between c-myc protein over-expression and c-myc gene amplification. These results may indicate that alteration of chromosomal regions on 8q and 17p, including c-myc and p53 genes, may be linked to progression of bladder cancer. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
