Functional restoration of human immunodeficiency virus and Epstein-Barr virus-specific CD8(+) T cells during highly active antiretroviral therapy is associated with an increase in CD4(+) T cells
| Autor: | Stefan, Kostense, Sigrid A, Otto, Gerlinde J, Knol, Erik H, Manting, Nening M, Nanlohy, Christine, Jansen, Joep M A, Lange, Marinus H J, van Oers, Frank, Miedema, Debbie, van Baarle |
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| Rok vydání: | 2002 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male Herpesvirus 4 Human Immunity Cellular Anti-HIV Agents HIV Antigens Macromolecular Substances Receptors Antigen T-Cell HIV Infections HIV Protease Inhibitors CD8-Positive T-Lymphocytes Viral Load Lymphocyte Activation Peptide Fragments HLA-B8 Antigen Cohort Studies Interferon-gamma T-Lymphocyte Subsets Antiretroviral Therapy Highly Active HLA-A2 Antigen HIV-1 Humans Reverse Transcriptase Inhibitors Lymphocyte Count |
| Zdroj: | European journal of immunology. 32(4) |
| ISSN: | 0014-2980 |
| Popis: | To investigate the effect of highly active antiretroviral therapy (HAART) on HIV- and Epstein-Barr virus (EBV)-specific CD8(+) T cells, total number and function of these cells was determined in 16 HIV-infected individuals using tetrameric HLA-peptide complexes and IFN-gamma ELISPOT assays after peptide stimulation, respectively. HAART induced a significant decrease in HIV-specific tetramer(+) T cells, whereas EBV-specific tetramer(+) T cells did not change. In addition, individuals who temporarily failed on therapy showed a temporary increase in the number of HIV-specific T cells, suggesting that differences in the pool size of antigen-specific T cells was determined by the presence of antigen. Interestingly, there was an increase in the ratio of IFN-gamma-producing T cells per total number of both HIV- and EBV-specific T cells in the majority of individuals, suggesting that the function of virus-specific T cells is improved in individuals successfully treated with HAART. Despite this relative functional improvement of EBV-specific T cells, no significant changes were observed in EBV load. In four subjects who temporarily failed on HAART, the percentage of IFN-gamma-producing T cells, both for HIV and EBV, paralleled CD4(+) T cell kinetics, suggesting that function seems to be related to differences in CD4(+) T cell numbers. Overall, these data indicate that HAART improves the antigen responsiveness of both HIV- and EBV-specific T cells, which is associated with an increase in CD4(+) T cells. |
| Databáze: | OpenAIRE |
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