| Autor: |
Carlos, Olano, Barrie, Wilkinson, César, Sánchez, Steven J, Moss, Rose, Sheridan, Vidya, Math, Alison J, Weston, Alfredo F, Braña, Christine J, Martin, Markiyan, Oliynyk, Carmen, Méndez, Peter F, Leadlay, José A, Salas |
| Rok vydání: |
2003 |
| Předmět: |
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| Zdroj: |
Chemistrybiology. 11(1) |
| ISSN: |
1074-5521 |
| Popis: |
The biosynthetic gene cluster for the angiogenesis inhibitor borrelidin has been cloned from Streptomyces parvulus Tü4055. Sequence analysis indicates that the macrolide ring of borrelidin is formed by a modular polyketide synthase (PKS) (borA1-A6), a result that was confirmed by disruption of borA3. The borrelidin PKS is striking because only seven rather than the nine modules expected for a nonaketide product are encoded by borA1-A6. The starter unit of the PKS has been verified as trans-cyclopentane-1,2-dicarboxylic acid (trans-1,2-CPDA), and the genes involved in its biosynthesis identified. Other genes responsible for biosynthesis of the nitrile moiety, regulation, and self-resistance were also identified. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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