Analysis of a C-methyltransferase gene (aviG1) involved in avilamycin biosynthesis in Streptomyces viridochromogenes Tü57 and complementation of a Saccharopolyspora erythraea eryBIII mutant by aviG1
| Autor: | G, Weitnauer, S, Gaisser, L, Kellenberger, P F, Leadlay, A, Bechthold |
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| Rok vydání: | 2002 |
| Předmět: |
DNA
Bacterial Base Sequence Molecular Structure Sequence Homology Amino Acid Genetic Complementation Test Molecular Sequence Data Oligosaccharides Methyltransferases Streptomyces Anti-Bacterial Agents Erythromycin Carbohydrate Sequence Genes Bacterial Mutation Amino Acid Sequence Gene Deletion Saccharopolyspora |
| Zdroj: | Microbiology (Reading, England). 148(Pt 2) |
| ISSN: | 1350-0872 |
| Popis: | Streptomyces viridochromogenes Tü57 is the principal producer of avilamycin A. aviG1, a putative methyltransferase gene, was detected in the avilamycin biosynthetic gene cluster. To determine the function of aviG1, a targeted gene inactivation experiment was performed. The resulting chromosomal mutant, carrying an in-frame deletion in aviG1, was deficient in avilamycin production. aviG1 was used to complement an eryBIII mutant of the erythromycin A producer Saccharopolyspora erythraea [Gaisser, S., Bohm, G. A., Doumith, M., Raynal, M. C., Dhillon, N., Cortes, J.Leadlay, P. F. (1998). Mol Gen Genet 258, 78-88]. The presence of erythromycin A in the culture supernatant of the complemented mutant indicated that L-mycarose biosynthesis could be restored and that AviG1 could take over the function of the C-methyltransferase EryBIII. |
| Databáze: | OpenAIRE |
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