| Autor: |
L Kh, Eĭdus, M O, Emel'ianov, A F, Korystova, L N, Kublik, V V, Shaposhnikova, Iu N, Korystov |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
Radiatsionnaia biologiia, radioecologiia. 50(1) |
| ISSN: |
0869-8031 |
| Popis: |
The effects of phorbol ether (PMA) and ionizing radiation on multidrug resistance (MDR) of human larynx cancer cells HEp-2 and the dependences of these effects on protein kinase C (PKC) activity and reactive oxygen species (ROS) production were studied. MDR was determined by transport rate of rhodamine 123 off cells and production of ROS in cells was measured by means of 2'7'-dichlorodigidrofuorescein oxidation to fluorescent 2',7'-dichlorofluorescein. ROS production was increased in cells at PMA treatment. This increase was caused by PKC dependent activation of NADPH-oxidase because the ROS increase suppressed completely with PKC and oxidase inhibitors. It was shown that tumor cell MDR was increased 16 h after PMA (100 nM) and radiation (1 Gy) treatment. The MDR increase depends on PKC activity and ROS increase in cells at both influences since PKC inhibitor and antioxidant, lipoic acid suppressed MDR increase. The obtained data are in accordance with hypothesis about the necessity of activation both signalization and stress reaction for initiation of transcription of transport protein genes which responsible for MDR of tumor cells. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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