A Novel GATA2 Protein Reporter Mouse Reveals Hematopoietic Progenitor Cell Types
| Autor: | Nouraiz, Ahmed, Leo, Kunz, Philipp S, Hoppe, Dirk, Loeffler, Martin, Etzrodt, Germán Camargo, Ortega, Oliver, Hilsenbeck, Konstantinos, Anastassiadis, Timm, Schroeder |
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| Rok vydání: | 2020 |
| Předmět: |
Aging
Neutrophils transcription factor networks Models Biological Monocytes Article Mice Genes Reporter GATA2 Animals fluorescent protein Cell Lineage Gene Regulatory Networks Mast Cells development Cell Proliferation cell fate Gene Expression Regulation Developmental Embryo Mammalian Hematopoietic Stem Cells monocyte and mast cell lineage Hematopoiesis GATA2 Transcription Factor hematopoietic stem and progenitor cell transgenic mouse Organ Specificity Transcription Factors |
| Zdroj: | Stem Cell Reports |
| ISSN: | 2213-6711 |
| Popis: | Summary The transcription factor (TF) GATA2 plays a key role in organ development and cell fate control in the central nervous, urogenital, respiratory, and reproductive systems, and in primitive and definitive hematopoiesis. Here, we generate a knockin protein reporter mouse line expressing a GATA2VENUS fusion from the endogenous Gata2 genomic locus, with correct expression and localization of GATA2VENUS in different organs. GATA2VENUS expression is heterogeneous in different hematopoietic stem and progenitor cell populations (HSPCs), identifies functionally distinct subsets, and suggests a novel monocyte and mast cell lineage bifurcation point. GATA2 levels further correlate with proliferation and lineage outcome of hematopoietic progenitors. The GATA2VENUS mouse line improves the identification of specific live cell types during embryonic and adult development and will be crucial for analyzing GATA2 protein dynamics in TF networks. Highlights • A novel GATA2VENUS fusion mouse line to report GATA2 protein expression • VENUS fusion does not alter GATA2 expression or disturb development or homeostasis • GATA2 expression identifies functionally distinct HSPC subpopulations • GATA2 expression unveils an earlier monocyte-mast cell lineage bifurcation point In this study, Schroeder and colleagues generated a GATA2VENUS protein reporter mouse line with normal GATA2 expression and localization, e.g., in the urogenital, auditory, and nervous systems. The authors also profiled GATA2 protein expression across hematopoietic cell types, identified heterogeneity within populations currently assumed to be homogeneous, and demonstrate an earlier monocyte-mast cell lineage bifurcation point. |
| Databáze: | OpenAIRE |
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