| Autor: |
Hong-Yin, Zhang, Jun, Chen, Jing, Jia, Dong, Liu, Lin-Chun, Shi, Hui, Zhang, Jing-Yuan, Song, Hui, Yao |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. 39(12) |
| ISSN: |
1001-5302 |
| Popis: |
In this study, the COI barcode was used to identify the Scolopendra medicinal materials and its adulterants in order to provide a new method for the identification of Scolopendra. Genomic DNA was extracted from the experimental samples. The COI sequences were amplified and sequenced bi-directionally. Sequence alignment and NJ tree construction was carried out by MEGA6.0 software. The results showed that the COI sequences can be obtained from all experimental samples. The average inter-specific K2P distance of Scolopendra was 0.222 and the minimum inter-specific distance was 0.190. All the Scolopendra subspinipes mutilans medicinal samples clustered into a clade in the NJ tree and can be distinguished from its adulterants. In a conclusion, COI can be used to correctly identify Scolopendra medicinal materials, and it will be a potential DNA barcode for identifying other animal medicinal materials. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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