Inhibition of arachidonic acid 5-lipoxygenase of human polymorphonuclear leukocytes by esculetin

Autor:	A G, Panossian
Rok vydání:	1984
Předmět:	Neutrophils Humans Arachidonic Acids Lipoxygenase Inhibitors Umbelliferones In Vitro Techniques Leukotriene B4 Chromatography High Pressure Liquid
Zdroj:	Biomedica biochimica acta. 43(12)
ISSN:	0232-766X
Popis:	Esculetin (6,7-dihydroxycoumarin) inhibits arachidonic acid 5-lipoxygenase of human polymorphonuclear leukocytes (HPMNL). Complete inhibition of the biosynthesis of 5-HETE, 5,12-DHETE and LTB4 was observed at a concentration of 100 microM (IC50 = = 0.4 microM, 6.0 microM and 0.1 microM, respectively). At these concentrations the formation of the cyclooxygenase product, 12-HHT, is increased. Upon stimulation of the cells with calcium ionophore A-23187, the inhibitory action of esculetin is revealed at the higher concentrations.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::50eea2764250fac00e5ee04b24203964 https://pubmed.ncbi.nlm.nih.gov/6100067 Zobrazit plný text záznamu