Microbial bile salt hydrolases mediate the efficacy of faecal microbiota transplant in the treatment of recurrent

Autor:	Benjamin H, Mullish, Julie A K, McDonald, Alexandros, Pechlivanis, Jessica R, Allegretti, Dina, Kao, Grace F, Barker, Diya, Kapila, Elaine O, Petrof, Susan A, Joyce, Cormac G M, Gahan, Izabela, Glegola-Madejska, Horace R T, Williams, Elaine, Holmes, Thomas B, Clarke, Mark R, Thursz, Julian R, Marchesi
Rok vydání:	2018
Předmět:	DNA Bacterial Clostridioides difficile Fecal Microbiota Transplantation Amidohydrolases Gastrointestinal Microbiome Mice Inbred C57BL Disease Models Animal Mice Recurrence Tandem Mass Spectrometry Clostridium Infections Animals Humans Female Glycocholic Acid
Zdroj:	Gut. 68(10)
ISSN:	1468-3288
Popis:	Faecal microbiota transplant (FMT) effectively treats recurrentUsing stool collected from patients and donors pre-FMT/post-FMT for rCDI, we performed 16S rRNA gene sequencing, ultra performance liquid chromatography mass spectrometry (UPLC-MS) bile acid profiling, BSH activity measurement, and qPCR ofFrom metataxonomics, pre-FMT stool demonstrated a reduced proportion of BSH-producing bacterial species compared with donors/post-FMT. Pre-FMT stool was enriched in taurocholic acid (TCA, a potentRestoration of gut BSH functionality contributes to the efficacy of FMT in treating rCDI.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::50aa942e3f647945bcdb809ff1e10b5a https://pubmed.ncbi.nlm.nih.gov/30837698 Zobrazit plný text záznamu